Food Protection Trends

Abstracts - July 2004

Validation of a Procedure Using CO2 for Rapid Cooling of Cheese Sauce

Acid and Alkaline pH Enhance Adhesion of Spores of  Alkaline-tolerant Bacillus cereus to Different Surface Types

Pilot-plant Evaluation of Acidified Sodium Chlorite for Sanitizing Beef Trim

Ethics of Differences  in Risk Perception  and Views on Food Safety


Validation of a Procedure Using CO2 for Rapid Cooling of Cheese Sauce

Peter Snyder, Jr.,* andRobin Mark Johnson
Hospitality Institute of Technology and Management, 670 Transfer Road, Suite 21A, St. Paul, MN 55114, USA; 16658 Hearthside Way, Lakeville, MN 55440, USA

SUMMARY
Cheese sauces are commonly produced in commercial kitchens. These sauces must be handled as potentially hazardous foods, unless challenge tests with Clostridium botulinum and Bacillus cereus are performed to demonstrate their microbial stability.  Although there are inhibitors in many cheese sauces (e.g., salt and exudates from lactic acid bacteria fermentations), the FDA 2001 Food Code requires that all potentially hazardous food be cooled from 140 to 70°F in 2 hours and from 70 to 41°F in 4 hours. Actually, this is a straight-line exponential cooling curve from 140 to 41°F in 6 hours.  Because cooling a large quantity of cheese sauce in 2-inch pans requires overnight refrigerated storage, during which time the sauce may be handled multiple times, the cooling process increases the risk of bacterial growth while placing a burden on the cooling capacity of refrigeration units. This study examined two cooling methods: cooling of cheese sauce in an ice bath followed by further cooling in refrigerated storage, and addition of CO2 (dry ice) to cheese sauce to cool it rapidly. 

The latter method of cooling was found to be more rapid, less labor intensive, and more cost effective. CO2 (dry ice) can also be used for cooling other potentially hazardous liquid or semi-liquid food products.


Acid and Alkaline pH Enhance Adhesion of Spores of  Alkaline-tolerant Bacillus cereus to Different Surface Types

D. Lindsay,2* V. S. Brözel,2 andA. von Holy1

1-School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Wits 2050, South Africa
2-Laboratory for Biofilm Physiology, Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria 0001, South Africa

SUMMARY
Few studies have addressed the effect of pH on adhesion of Bacillus (B.) spores to surfaces.  In this study, spore adhesion of B. cereus DL5, isolated from alkaline dairy wash solutions, and B. cereus NCTC 2599 was studied at pH 4, 7 or 10 on stainless steel, glass and polyurethane surfaces. Spores of each strain were suspended in sterile distilled water adjusted to pH 4, 7 or 10 and stored at 4°C overnight. Stainless steel, glass and polyurethane surfaces that had been conditioned with one-tenth strength Tryptone Soya Broth were then exposed to the spore suspensions at each pH value for 1 hour.  Adhering spores were enumerated by the standard plate count method at all pH values. Results indicated that pH had little effect on adhesion of B. cereus NCTC 2599 spores to any of the surface types.  By contrast, B. cereus DL5 spores showed enhanced adhesion at pH 4 and 10 to polyurethane and glass surfaces.  Acid or alkaline pH values in food processing environments may exist on food contact surfaces after cleaning-in-place procedures, such as in dilute cleaning solution residues overnight.  The adhesion of spores of Bacillus spp. to food contact surfaces constructed of materials such as stainless steel and polyurethane is already a concern for food manufacturers.  Thus, enhanced spore adhesion potential of B. cereus strains under certain pH conditions may have important spoilage and safety implications.


Pilot-plant Evaluation of Acidified Sodium Chlorite for Sanitizing Beef Trim

K. R. SCHNEIDER,1* C. C. WARF,2 P. L. BAXTER,3 B. R. WARREN,1 and G. K. KEMP2
1-University of Florida, Department of Food Science and Human Nutrition, 359 FSHN Bldg., Newell Dr., Gainesville, FL 32611, USA 2-Alcide Corporation, 8561 154th Ave. N.E., Redmond, WA 98052, USA
3-Florida Department of Agriculture and Consumer Services, 3125 Conner Blvd., Bldg. 9, Tallahassee, FL 32399, USA

SUMMARY
Citric acid activated acidified sodium chlorite (C-ASC) has been approved for sanitizing beef trim. In this study, the efficacy of 1000 ppm C-ASC spray treatments against total aerobic microorganisms and E. coli was evaluated while optimizing a newly developed SANOVA® system for spraying beef trim.  Spray treatments consisted of combinations of 74, 147, or 221 ml C-ASC/kg delivered during 5, 10, or 15 s.  Additionally, two feed rates, 0.75 kg/s and 2.5 kg/s, were investigated. Reductions by C-ASC spray treatments on 90% lean/10% fat [90/10] beef trim were compared to reductions by the same treatment on 50% lean/50% fat [50/50] beef trim.  At a feed rate of 0.75 kg/s, the SANOVA® system reduced total aerobic microorganisms by as much as 1.2 log CFU/50 cm2 (treatment with 221 ml C-ASC/kg for 10 s) and reduced E. coli by as much as 1.4 log CFU/50 cm2 (treatment with 221 ml C-ASC/kg for 15 s). At a feed rate of 2.5 kg/s, the SANOVA® system reduced total aerobic microorganisms by as much as 1.3 CFU/50 cm2  (treatment with 221 ml C-ASC/kg for 15 s) and reduced E. coli by 2.3 CFU/50 cm2  (treatment with 147 ml C-ASC/kg for 10 s). Reductions of both aerobic microorganisms and E. coli were higher at feed rates of 2.5 kg/s than at 0.75 kg/s for most matrices tested. Preliminary evaluations suggest that higher reductions may be possible on [50/50] beef trim than on [90/10] trim.


Ethics of Differences  in Risk Perception  and Views on Food Safety

Michiel Korthals
Wageningen University, The Netherlands, Hollandseweg 1, 6706 KN Wageningen

SUMMARY
Conventional risk analysis presupposes that uniform definitions of risks can be reached on the basis of scientific consensus; it does not take consumers’ definitions of risks seriously. However, risk definitions can vary widely, depending on national cultures and their influence on scientific communities. In addition, risks must be accepted by members of society, both individually and jointly.  The issue is then no longer one of costs and benefits, but of mutual respect of rights and of achieving trust and reasonable agreement among members of society. In this paper the controversies between scientific and cultural risk perception will be considered, and the issue of the ethical acceptability of different risk definitions. A key element for consumers is trust in the authority that defines and sets out the risks for policy purposes, and trust is not upheld by dismissing their definitions as irrational.  For consumers, but for scientists as well, cultural background, basic assumptions, expectations, and lifestyles play a major role here. Subsequently, the ethical legitimation of pluralism in risk perception is discussed.  Consumers not only have money to buy products but hold varying views about freedom of food choice and diversity of the food supply. In democratic societies, legitimately developed risk definitions of consumers are entitled to recognition, and such definitions cannot be set aside by scientific, free-market, or utilitarian considerations (such as cost-benefit analyses prepared by others). Implications for risk assessment and risk communication are explained in detail.