Food Protection Trends

Abstracts - December 2005

The Microbiological Status of Non/Food Contact Surfaces in Domestic Kitchens and the Growth of Staphylococcus aureus in Domestic Refrigerators

Factors Impacting Food Workers’ and Managers’ Safe Food Preparation Practices: A Qualitative Study

Color of Low Dose-Irradiated Ground Beef Before and After Cooking to 60°C or 71°C and Survival of E. coli O157:H7 in Irradiated Patties


The Microbiological Status of Non/Food Contact Surfaces in Domestic Kitchens and the Growth of Staphylococcus aureus in Domestic Refrigerators

J. Kennedy-1, I. S. Blair-2, D. A. McDowell-2, and D. J. Bolton-1*

1-Foods Safety Department, Teagasc – The National Food Centre, Ashtown, Dublin 15, Ireland
2 Food Microbiology Research Group, NICHE, University of Ulster at Jordanstown, N. Ireland


SUMMARY
The objectives of this study were to investigate the incidence/levels of bacterial contamination at key sites in domestic kitchens and to assess the potential for Staphylococcus aureus growth during domestic chilled storage. Domestic kitchen surfaces and dishcloths were examined for total viable count (TVC), total Enterobacteriaceae count (TEC), total coliform count (TCC) and the presence/absence of Salmonella spp., Campylobacter spp., Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus and Escherichia coli O157:H7. The patterns of fluctuation in refrigeration air temperatures over 7 days were recorded and used to model the potential growth of S. aureus in broth. On the kitchen surfaces the TVCs varied between 1.8 log 10 cfu cm -2 (microwave) and 5.8 log 10 cfu cm -2 (refrigerator). TECs varied between 0 log 10 cfu cm -2 (microwave) and 2.1 log 10 cfu cm -2 (sink). TCCs ranged from 0.9 log 10 cfu cm -2 (microwave) to 3.0 log 10 cfu cm -2 (sink). The dishcloths contained higher total counts than any surface examined and were also a source of E. coli, L. monocytogenes and S. aureus. The average air temperature in domestic refrigerators varied from 4.6˚C to 6.4˚C, while in the refrigerator with the highest temperature profile, the temperature varied from 11.4˚C to 12.2˚C. Growth studies indicated that S. aureus numbers increased by approximately 3.7 log 10 cfu cm -2 during storage for 7 days at the observed highest temperature profile. Modelling this data by use of the Monod equation suggested a generation time of approximately 10 h during the exponential growth phase at these temperatures, suggesting that microgram levels of toxin may be present after 7 days. This study reinforces the need for information regarding adequate cleaning, prevention of cross contamination and effective cold storage to prevent acquisition and transmission of infection in the home.

Factors Impacting Food Workers’ and Managers’ Safe Food Preparation Practices: A Qualitative Study

Laura R. Green-1*, and Carol Selman-2

1-Health, Social, and Economics Research, RTI International, Research Triangle Park, NC, USA
2-Environmental Health Services Branch, Division of Emergency and Environmental Health Services,
National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA, USA

SUMMARY
This study collected data on food workers’ self-reported food safety practices and beliefs about factors that impacted their ability to prepare food safely. Eleven focus groups were conducted with food service workers and managers in which they discussed their current implementation of seven food preparation practices (handwashing, hot holding, etc.), and the factors they believed impacted their safe implementation of those practices. Some participants reported unsafe food preparation practices, such as inappropriate glove use and not checking the temperatures of cooked, reheated, and cooled foods. Most participants, however, reported safe practices (e.g., washing their hands after preparing raw meat). Participants identified a number of factors that impacted their ability to prepare food safely, including time pressure; structural environments, equipment, and resources; management and coworker emphasis on food safety; worker characteristics; negative consequences for those who do not prepare food safely; food safety education and training; restaurant procedures; and glove and sanitizer use. Results suggest that food safety programs need to address the full range of factors that impact food preparation behaviors.


Color of Low Dose-Irradiated Ground Beef Before and After Cooking to 60°C or 71°C and Survival of E. coli O157:H7 in Irradiated Patties

Michael D. J. Peirson-1, Donna Ryland-2 & 3, and Richard A. Holley-1*
1-Department of Food Science, Faculty of Agricultural & Food Sciences, University of Manitoba, Winnipeg, MB R3T 2N2 Canada
2-Department of Human Nutritional Sciences, Faculty of Human Ecology, University of Manitoba, Winnipeg, MB R3T 2N2 Canada
3-Present Address: Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Road, Winnipeg, MB R3T 2M9 Canada

SUMMARY
The effects of electron beam irradiation (1.5 or 2 kGy), vacuum packaging, and end-point temperature (60°C or 71°C) on color of fresh and frozen ground beef were examined. The effect of irradiation on survival of Escherichia coli O157:H7 was also examined. Irradiation caused aerobically packaged ground beef to become permanently less red, but irradiated vacuum-packaged fresh ground beef suffered only temporary browning and regained its original redness when exposed to air. Average HunterLab ‘a’ and ‘b’ values were lower for meat patties cooked to 71°C than for meat cooked to 60°C. In cooked patties, the fresh-frozen, vacuum-aerobic, treatment group combinations could not be consistently separated by HunterLab colorimetry. A sensory panel found that patties made from frozen irradiated ground beef appeared fully cooked at only 60°C, but that patties of fresh vacuum-packaged irradiated ground beef appeared similar to non-irradiated patties. In vacuum-packed fresh patties, irradiation (1.1 kGy) killed ≤ 2.9 log 10 cfu/g of E. coli O157:H7.