Food Protection Trends

Abstracts - January 2007

Effect of Cooling Rate on Pathogen Survival in Post-Process Contaminated Yogurt
 

Controlling Listeria monocytogenes in a Retail Setting 


Effect of Cooling Rate on Pathogen Survival in Post-Process Contaminated Yogurt

Kathleen A. Glass, Lindsey M. McDonnell, Rob C. Rassel, and Kristine L. Zierke

Food Research Institute, University of Wisconsin-Madison, 1925 Willow Drive, Madison, WI 53706, USA

SUMMARY
The effect of cooling rate on the survival of pathogens was compared in full-fat and nonfat yogurt with strawberry fruit preparation added (final pH 4.4). Products were inoculated with 4.5 log CFU/g of acid-adapted Listeria monocytogenes, Salmonella sp., or Escherichia coli O157:H7 and dispensed into yogurt cups. One set of packages was immediately chilled to 7.2°C, whereas a parallel set of packages was cooled from 27°C to 7.2°C within 96 hours, and then both sets were stored at 7.2°C for the duration of the two-week study. Triplicate samples of each treatment were enumerated by standard plating methods at 24, 48, 72, 96, 168, 240, and 336 hours storage. In yogurt cooled slowly, populations decreased 1.3 to 1.7 logs for Listeria, 2.2 to 3.0 logs for Salmonella, and 0.8-log for E. coli O157:H7 within 96 hours. In contrast, log reductions were 0.5 for Listeria, 1.7 to 1.9 for Salmonella, and 0.2 to 0.3 log for E. coli O157:H7 in yogurt chilled immediately and held at 7.2°C for 96 hours. The pH decreased in all yogurt treatments during the 2-week storage, but the decrease was more rapid in the yogurt cooled slowly (final pH 4.1) than in samples chilled to 7.2°C immediately after inoculation (final pH 4.2). These data support the safety implications of filling yogurt with active cultures at 27°C followed by cooling to < 7.2°C within 96 hours. To ensure a safe product to the consumer, manufacturers should also comply with good manufacturing practices and environmental controls.

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Controlling Listeria monocytogenes in a Retail Setting

Dale A. Grinstead-1* and Catherine N. Cutter-2

1-JohnsonDiversey, 8310 16th St., Sturtevant, WI 53177
2-Dept. of Food Science, 202 Food Science Bldg., Pennsylvania State University, University Park, PA 16802

SUMMARY
Listeria monocytogenes (LM) is a deadly pathogen that sickens approximately 2,500 people in the United States each year and has a mortality rate of approximately 20%. The serious nature of the illnesses caused by this organism makes control of LM in any food handling environment important. Over the past decade, a vast amount of data has been published about LM, including the illness it causes, the nature of the organism, and its ecology. However, very little information has focused on LM in a retail environment. Recent research has demonstrated that LM is associated with a variety of ready-to-eat foods produced in retail settings. It is likely to be found in the retail environment, and there are many locations that could become harborage points for the pathogen. The most effective way to control LM in foods may be to keep the LM population to less than 100 cells per gram of food. That means that it is important to control the growth of this pathogen in food and in retail and other food handling environments. Fortunately, there are some steps that can be taken to help prevent LM from becoming established in a retail facility. These steps include proper training of employees and operators, keeping
the retail environment as dry and clean as possible, maintaining temperature controls, and ensuring that employees follow good personal hygiene practices. Although L. monocytogenes is a dangerous organism, it is possible to control the organism and minimize the risks that it presents in a retail setting.

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