Dairy, Food and Environmental Sanitation

Abstracts - March, 2001

Building Product Confidence with Challenge Studies

An Improved Test Method for Evaluating Barrier Teat Disinfectants


Building Product Confidence with Challenge Studies
Ellen M. Vestergaard*
Silliker Laboratories, Corporate Research Center, 160 Armory Drive, South Holland, IL 60473

Summary

The shelf life and stability of food products are of concern to processors, retailers, and consumers. A challenge study, in which microorganisms are inoculated into a formulation, is a useful tool for determining the risk of spoilage or growth of pathogens. The study parameters are based upon an evaluation of the food and its storage conditions. The selection of appropriate challenge organisms, duration of the study, level of inoculum, storage temperature, and packaging of samples must all be considered. The data obtained help processors develop optimal product formulations, storage, and packaging.

An Improved Test Method for Evaluating Barrier Teat Disinfectants
G. K. Kemp
Alcide Corporation, 8561 - 154th Ave., Redmond, WA 98052

Summary

The National Mastitis Council experimental challenge procedure was adapted for the evaluation of effectiveness of acidified sodium chlorite (ASC) barrier teat disinfectants against the environmental pathogen Streptococcus uberis. ASC barrier disinfectants that were applied immediately after milking were tested for their ability to protect against mastitis resulting from application of a challenge inoculum one hour before milking. In a pair of 8- and 10-week studies, two of three ASC postmilking germicidal barrier teat disinfectants, activated by mandelic acid, significantly reduced new intramammary infections (59.1% and 68.4%, at 8 and 10 weeks, respectively). These reductions were equivalent to that obtained by a combined pre- and post-dipping treatment using a commercial 0.5% iodine disinfectant (58.3%) and were in contrast to the reduction obtained with a lactic acid-activated counterpart (35.0%).