Standardized Biofilm Methods for Laboratory Studies of Biofilms

Workshop Registration Fees

  • Members TBD
  • Non-Members TBD
  • Student TBD
  • Late-fee TBD

Workshop Description

Standard methods development is the creation of laboratory protocols for the purpose of comparison, both within a single laboratory and among various laboratories. Researchers choose to use a standard method for various reasons. For instance, a standard method is useful for teaching proper laboratory protocol or monitoring equipment performance. The impetus for the development of many microbial standard methods, however, is efficacy testing for product registration with a regulatory agency such as the US Environmental Protection Agency (EPA) or the US Food and Drug Administration (FDA). The mission of the Standardized Biofilm Methods Laboratory at the Center for Biofilm Engineering is the development and validation of biofilm methods for growing, treating, sampling and analyzing biofilm bacteria. The biofilm growth methods presented will include the CDC biofilm reactor (ASTM Method E2562), drip flow biofilm reactor (ASTM 2647) and MBEC Assay (ASTM Method 2799). The treatment methods presented will include the Single Tube Method (ASTM Method 2871) and the MBEC method (ASTM Method 2799). During this workshop, participants will learn each component of the three biofilm growth methods and critical parameters of each component. Emphasis will be on selecting a reactor system to grow a biofilm representative of a particular environment of interest and considerations for modifying the standards to other microorganisms. Small group activities will allow the participants to work hands-on with the reactors and to ask specific questions of the instructors. During the biofilm analysis portion of the workshop, the statistical attributes of repeatability, reproducibility, responsiveness and ruggedness of a standard method will be demonstrated using recent multiple-laboratory study results.

Anyone interested in standard methods, growing relevant biofilms, and those who wish to test surfaces, agents or fabrics for antimicrobial efficacy against biofilms.

Workshop Topics

An Introduction to Biofilms and Laboratory Growth Reactors

Adapting Standardized Biofilm Growth Methods for Other Bacteria

Important Considerations for Lab Studies with Biofilm –

Keepin’ it Real: The Statistical R’s of Biofilm Methods

Visualizing Treatment Impacts on Biofilm

Now You’ve Got It, What Do You Do With It

Workshop Instructors

Diane Walker, MSU Center for Biofilm Engineering, Bozeman, MT

Kelli Buckingham-Meyer, MSU Center for Biofilm Engineering, Bozeman, MT

Lindsey Lorenz, MSU Center for Biofilm Engineering, Bozeman, MT

Albert Parker, MSU Center for Biofilm Engineering, Bozeman, MT

Workshop Organizers

Diane Walker, MSU Center for Biofilm Engineering, Bozeman, MT