Workshop 1 - Next Generation Sequencing: A Tutorial and Hands-on Workshop to Help Understand This Emerging Technology

Workshop Registration Fees

  • Members $685.00
  • Non-Members $785.00
  • Student $342.50
  • Late-fee $100.00

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LATE FEE BEGINS June 28

Cancellation Policy:  Registration fees, less a $100 administration fee and any applicable bank charges, will be refunded for written cancellations received by July 7, 2022.  No refunds will be made after July 7, however, the registration may be transferred to a colleague who is NOT currently registered with written notification.  Registrations cannot be carried to any future meetings.

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Other:  Any modifications to payment method will incur a $25 processing fee.

Refunds will be processed when received.

Workshop Description

Next Generation Sequencing (NGS) has taken the Front Stage as a tool to understand the environment around us.  It is being used globally to track outbreak strains of bacteria, monitor microbial communities and understand changes in populations of organisms based on temporal and forced stimuli.  NGS is more complex than past methodologies (such as PFGE) and has more components that need to be understood. What is NGS? How do I perform an experiment? How do I analyze my data?  What does the data mean? What is metagenomics and how can I analyze a metagenomic data set? And what does Precision Genomics mean? This workshop seeks to shed light on these questions so that the student will have a more holistic view of the applications of NGS. We will provide sessions on technology, data analysis and data interpretation that the FDA, CORE, Compliance and CDC, employ for outbreak investigations and regulatory decision-making.  Each attendee will be analyzing whole genome sequencing and metagenomic datasets to perform quality control, assemble, build phylogenetic trees, and identify genes of interest (such as AMR, virulence and stress response genes) utilizing open source tools such as GalaxyTrakr, Pathogen Detection Website and the Center for Genomic Epidemiology Database. Upon return from the workshop the knowledge gained and the tools learned can be implemented for data analysis in your lab.

Who Should Attend

Industry, Academia, students, government.

Workshop Topics

  • Introduction to NGS Techniques
  • Introduction to Metagenomics
  • Introduction to WGS Data Analytics
  • Introduction to Phylogenetics Introduction to GenomeTrakr and Meta Data Introduction to Pathogen Detection Website
  • WGS Applications for Foodborne Illness Outbreak Investigation
  • WGS in Regulatory Decision Making
  • Introduction to a Bioinformatic Computing Environment
  • Hands-On Analysis

Workshop Instructors

  • Maria Balkey, U.S. Food and Drug Administration – Center for Food Safety and Applied Nutrition, College Park, MD, College Park, MD, USA
  • Laura Gieraltowski, CDC, Atlanta, GA, USA
  • Julie Haendiges, U.S. Food and Drug Administration – Center for Food Safety and Applied Nutrition, College Park, MD, USA
  • Leslie Hintz, U.S. Food and Drug Administration, College Park, MD, USA
  • Maria Hoffmann, U.S. Food and Drug Administration – Center for Food Safety and Applied Nutrition, College Park, MD, USA
  • Bill Klimke, NCBI, Washington, DC, USA
  • Padmini Ramachandran, U.S. Food and Drug Administration, Laurel, MD, USA
  • Eric Stevens, U.S. Food and Drug Administration, College Park, MD

Workshop Organizers

  • Maria Hoffmann, U.S. Food and Drug Administration – Center for Food Safety and Applied Nutrition, College Park, MD, USA
  • Eric Stevens, U.S. Food and Drug Administration, College Park, MD, USA